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Wednesday, March 10, 2010

Bacillus stearothermophilus

The nutrient broth tubes of B. stearothermophilus I made last week show no discernable growth in an incubator set at 37 degrees C. So, today I streaked some nutrient agar plates with broth from those tubes as well as from the original agar slant that it came on from Carolina last week. Those slants have also been stored at 37C. I'm hoping elevating the temperature to 60-65 degrees C will promote growth, so I've uped the temp in one of the incubators. We'll see if that does anything. The only other issue is that I noticed the slants are actually on tryptic soy agar, so I'm not even sure B. stearothermophilus will grow on nutrient agar. Time will tell, I guess.

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