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Tuesday, March 23, 2010

Thioglycollate broth is really cool!


As you can see, the thioglycollate broth, which has a color indicator identifying oxygen level within the broth, turned out quite nicely. Thioglycollate broth has a low oxidation- reduction potential and therefore contains litte free oxygen. The pinkish/blue region at the top has more oxygen in it and the clearish yellow region at the bottom is almost oxygen free. The students inoculated their tubes yesterday with E. coli, Lactococcus lactis, and Pseudomonas fluorescens. E. coli is facultatively anaerobic and will grow best at the bottom of the tube, but will have good growth throughout. P. fluorescens is aerobic and should only grow at the top of the tube (if their cultures were clean). Lactococcus lactis is fairly anaerobic and should grow best at the bottom of the tube, but may exhibit some growth farther up the tube.

Oh, btw, Lactococcus did not grow at all on nutrient agar, so here is the email message I received from Dr. McPherson about a growth medium for it:

This medium is called Corynebacterium Agar, but it can be used for the
cultivation of L. lactis.

Agar 15 g
Tryptic digest of casein 10 g
Glucose 5 g
NaCl 5 g
Yeast extract 5 g
dH2O 1000 ml

pH 7.2-7.4 at 25 degrees Celsius

The formulation of Tryptic Soy Agar is

Agar 15 g
Pancreatic digest of casein 5 g
Papaic digest of soybean 5 g
NaCl 5 g
dH2O 1000 ml

pH 7.1-7.5 at 25 degrees Celsius

The formulation of Nutrient Agar is

Agar 15 g
Beef extract 3 g
Peptone 5 g
dH2O 1000 ml

pH 6.6-7.0 at 25 degrees Celsius

So, TSA is closer in formulation to the Corynebacterium agar.
If you don't have all the components to make the Corynebacterium agar,
you could try adding a little glucose to TSA.

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